Aktivitas Daya Hambat Isolat Actinomycetes dengan Lama Fermentasi yang Berbeda terhadap Pertumbuhan Klebsiella pneumoniae

Actinomycetes are filamentous, Gram-positive bacteria widely distributed in soil and can produce secondary metabolites useful as antibiotics. The synthesis of is influenced by the growth conditions each isolate, including fermentation period. Klebsiella pneumoniae an opportunistic bacterial pathogen causing lower respiratory tract infection. Objectives: To determined inhibition activity with different periods against K.pneumoniae. Methods: were isolated from Kebun Raya Bogor grown on Starch Casein Agar (SCA) medium before being fermented liquid media consisting 2% mannitol, peptone, 1% glucose incubation time 6,7, 8 days. An antibacterial test was conducted Mueller Hinton (MHA) using well diffusion method. Results: isolates duration 6,7 able to inhibit K. average zone 4,46 mm; 4,94 5,04 mm, respectively. Actinomycetes' inhibitory mechanism ranges cell wall nucleic acid protein synthesis. One Way ANOVA showed significant differences between produced group. Conclusion: have highest achieved a days.Keywords: Actinomycetes, antibacterial, fermentation,